What is the role of DNA ligase in the process of genetic engineering where DNA fragments are cut by restriction enzymes?

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To cut DNA into fragments

To separate DNA fragments based on size

To join together DNA fragments with complementary 'sticky-ends'

To replicate DNA fragments

answer is B.

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Detailed Solution

The DNA ligase enzyme family facilitates the creation of a phosphodiester bond between adjacent 5'-phosphate and 3'-hydroxyl termini in double-stranded DNA. This function enables the sealing of breaks in double-stranded DNA or the merging of DNA fragments with either blunt or cohesive ends.

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