What staining technique is used to locate lysosomes?

Concept: Staining is a microscopy technique for enhancing contrast in a microscopic picture.

Stains and dyes are widely used to highlight features in microorganisms for viewing, which is often done using a variety of microscopes.

Using acridine orange as a lysosomal indicator, fluorescent experiments indicated the existence of spherical, reddish-orange fluorescing entities of varied sizes. Acid phosphatase (AP), a lysosome flag enzyme, was found in single-membrane attached organelles using electron microscopy.