When a biochemical reaction is carried out in laboratory in the absence of enzyme then rate of reaction obtained is 10- times, then activation energy of reaction in the Presence of enzyme is

The presence of enzyme (catalyst) increases the speed of reaction by lowering the energy barrier, i.e. a new path is followed with lower activation energy. 

Here E_T, is the threshold energy. 
${\mathrm{E}}_{\mathrm{a}}\text{ and }{\mathrm{E}}_{{\mathrm{a}}_1}$is energy of activation of reaction in absence and presence of catalyst respectively.