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When a desirable gene is inserted into the recognition site of Cla I of PBR₃₂₂ and is introduced to a host bacterium, then the host bacterium will

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Loose its resistance to tetracycline only

Loose its resistance to ampicillin only

Remain sensitive to both ampicillin and tetracycline

Become resistant to both tetracycline and ampicillin

answer is D.

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Detailed Solution

Since Cla I site on the vector PBR322 does not contain any of the selectable marker genes,the vector remains resistant to both ampicillin and tetracycline .

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