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When a pBR322 plasmid is treated with Bam HI and successfully receives a foreign gene insert, the resulting bacterial colony exhibits resistance to antibiotic A and sensitivity to antibiotic B. Identify antibiotics A and B respectively from the options below:

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Kanamycin & Ampicillin

Streptomycin & Kanamycin

Ampicillin & Tetracyclin

Tetracyclin & Ampicillin

answer is A.

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Detailed Solution

BamHI is located within tetracycline gene,so when a foreign gene is inserted at this location tet gene undergoe sinsertional inactivation.Thus the resultant vector is resistant to ampicillin but sensitive to tetracycline.

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