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Q.

When a PBR322 plasmid is treated with Bam HI to clone a foreign insert successfully, then the colony would be resistant to _A_ and sensitive to _B_. What are A and B ?

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a

Ampicillin and tetracyclin

b

Tetracyclin and ampicillin

c

Ampicillin and kanamycin

d

Kanamycin and ampicillin

answer is A.

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Detailed Solution

Bam HI recognition sequence is present at tetacyclin resistant gene hence resistance to this antibiotic is lost when a desired gene get attached at this place

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