When and why are glycerine and safranin used in the experiment to prepare a temporary mount of a leaf peel to show stomata? Explain.

Glycerine is used just league placing the specimen on the slide. It helps it to keep moist and prevent drying. Safranin is a synthetic dye used to stain the cells of the leaf so that each part is visible properly. It is applied after the leaf peel is washed in water and kept in a watch glass.