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Q.

Which amongst the following is employed to check the progression of a restriction enzyme digestion?

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a

Sodium dodecyl sulfate polyacrylamide gel electrophoresis

b

Agarose gel electrophoresis

c

Isoelectric focusing

d

Immunochemical electrophoresis

answer is B.

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Detailed Solution

Agarose gel electrophoresis is employed to check the progression of a restriction enzyme digestion. Since DNA fragments are negatively charged molecules, they can be separated by forcing them to move towards the anode under an electric field through a medium/matrix.

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