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Which amongst the following statement is true about restriction endonucleases?

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They can cut DNA strands randomly at any base sequence.

They can bind to and cleave only single stranded DNA.

They generally do not have any role to play in gene cloning.

They often are known to generate cohesive ends in the DNA fragments formed by their action.

answer is D.

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Detailed Solution

The restriction endonucleases often are know to generate long overhangs which are known as sticky ends or cohesive ends.
They cleave DNA at specific sequence sites along the molecule.
They have been isolated mainly from bacteria.

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