Which are the various processes in tissue culture?

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Detailed Solution

The main processes involved in tissue culture are:

Selection of explant – Choosing a small piece of plant tissue (like leaf, stem, or root).
Sterilization – Cleaning explant and equipment to remove microorganisms.
Preparation of nutrient medium – A culture medium (with minerals, vitamins, hormones, and sucrose) is prepared.
Inoculation – Placing the sterilized explant onto the nutrient medium under aseptic conditions.
Incubation – Keeping the culture under controlled light, temperature, and humidity for growth.
Regeneration – Cells divide and differentiate to form callus, shoots, and roots.
Hardening and transfer – Plantlets are gradually adapted to natural conditions and transferred to soil.

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