Which enzyme joins the gene of interest and cut vector?

Ligase is responsible for joining or ligating the DNA fragments together by catalyzing the formation of phosphodiester bonds between adjacent nucleotides. In the context of recombinant DNA technology, ligase is used to join DNA molecules from different sources. For example, in the process of creating recombinant DNA, the gene of interest, which has been cut out from the source DNA using a restriction enzyme, is joined or ligated into a vector DNA.