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Q.

Which of the following are correct statement?
(i) Polyethylene glycol method is used for gene transfer without vector.
(ii) Biolistic gun is a suitable method for transferring gene into animal cell.
(iii) plasmid is a single-stranded DNA.
(iv) In recombinant DNA technology, cDNA is prepared from mRNA.

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a

(i) and (ii)

b

(ii) and (iii)

c

(i) and (iv)

d

(ii) and (iv)

answer is C.

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Detailed Solution

Polyethylene glycol method is used for direct gene transfer into host cell without vector. cDNA is prepared from mRNA by reverse transcription and it is used as probe in identifying desired gene or recombinants. 

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