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Which of the following assumptions is necessary to theoretically calculate the net gain of ATP from one glucose molecule during aerobic respiration?

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Intermediates from the pathways are frequently utilized to synthesize other compounds.

The pathways are assumed to function sequentially, and NADH enters the mitochondria

Glycolysis, TCA cycle, and ETS pathway occur randomly, not sequentially.

NADH produced during glycolysis is not transferred into the mitochondria.

answer is D.

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The pathways are assumed to function sequentially, and NADH enters the mitochondria.

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