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Which of the following is a correct sequence of processes in recombinant DNA technology?
I. Transfer of the recombinant DNA into the host.
II. Isolation of DNA with desirable genes.
III. Introduction of identified DNA into the vector
IV. Downstream processsing
V. Culturing the host cells at large scale in bioreactor.

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I → II → III→ IV → V

II → III → I → V → IV

III → I → II → IV → V

I → III → II → V → IV

answer is B.

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Detailed Solution

The correct sequence is:

Isolation of DNA fragment or desirable gene

Introduction of gene into the the vector to form rDNA.

Transfer of the recombinant DNA into the host.

Culturing the host cells at large scale in bioreactor.

Downstream processing.

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