Q.

Which of the following is a thermostable enzyme?

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a

Reverse transcriptase

b

RNA polymerase

c

Taq polymerase

d

Carbonic anhydrase

answer is C.

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Detailed Solution

In a polymerase chain reaction, a segment of DNA can be amplified approximately a billion times, i.e., 1 billion copies are made. Such repeated amplification is achieved by the use of a thermostable DNA polymerase (isolated from a bacterium, Thermus aquaticus), which remains active during the high temperature-induced denaturation of double-stranded DNA.

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