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Which of the following is an incorrect statement regarding the enzymes used for recombinant DNA technology?

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DNA ligase helps in sealing gaps and acts as molecular glues.

Reverse transcriptase is used to from cDNA from mRNA.

DNA polymerase removes phosphate groups from the 5' end of double-stranded DNA and prevents unwanted ligation.

Restriction endonucleases open the vector by breaking the phosphodiester bond between two nucleotides of a specific sequence.

answer is C.

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Detailed Solution

DNA polymerases do not remove phosphate groups from 5' end of nucleic acid strands. They usually attach nucleotides to 3' end of the preexisting nucleotides over the template.

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