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Q.

Which of the following is an incorrect statement with respect to PCR technique?

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a

Two oligonucleotide primers anneal to each of the two strands of DNA at their 3' complimentary ends.

b

Deoxyribonucleotide triphosphates are used raw material for the synthesis of new DNA strand.

c

A 17-25-nucleotides-long primer is needed to bind with the template.

d

The optimum temperature for polymerisation is 90oC

answer is B.

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Detailed Solution

In PCR, the optimum temperature maintained during extension stage for Taq polymerase activity is around 900C.

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