Which of the following is correct w.r.t. PCR?

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Denaturation at 48°C, separating the two DNA strands

Extension of primers at 98°C

Extension in the presence of heat stable DNA polymerase

Annealing with two sets of primers at 72°C

answer is C.

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Detailed Solution

Denaturation at 94°C - 96°C, separating the two DNA strands.
Annealing of primers occur at 50°-60° C.
Extension in the presence of heat stable DNA polymerase (Taq polymerase) at 72°C.

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