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Q.

Which of the following is found in acid soluble pool?
 

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a

Nucleic acid 
 

b

Lipids 
 

c

Amino acids 

d

Polysaccharides  
 

answer is D.

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Detailed Solution

Acid hydrolysis of any living tissue (a vegetable or a piece of liver, etc.) involves grinding the tissue with trichloroacetic acid (Cl3CCOOH) using a mortar and a pestle. We obtain a thick slurry. If we were to strain this through a cheesecloth or cotton we would obtain two fractions. One is called the filtrate or more technically, the acid-soluble pool, and the second, the retentate or the acid-insoluble fraction. Scientists have found thousands of organic compounds in the acid-soluble pool. The molecular weights of these biomolecules are less than 800 Da, and they come under acid soluble fraction.

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