Which of the following is incorrect way to introduce alien DNA into host cells ?
i) Bacteria is made ‘competent’ to take up DNA by treating them with a specific concentration of a divalent cation, such as calcium.
ii) Recombinant DNA is transfer into competent bacteria by incubating the cells with recombinant DNA on ice, heat shock, and then putting them back on ice.
iii) Recombinant DNA is directly injected into the nucleus of plant cell by micro-injection.
iv) Recombinant DNA is directly injected into the nucleus of an animal cell by biolistics or gene gun.
v) ‘Disarmed Pathogen’ vectors, when allowed to infect the cell, transfer the recombinant DNA into the host.

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i and ii

ii and iii

iii and iv

iv and v

answer is C.

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Detailed Solution

In a method known as micro-injection, recombinant DNA is directly injected into the nucleus of an animal cell. In another method, suitable for plants, cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA in a method known as biolistics or gene gun.

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