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Q.

Which of the following is not a characteristic feature of the vector pBR322?

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a

It has two antibiotic - resistance genes : ampR and tetR.

b

It was the first artificial cloning vector constructed in 1997 by Boliver and Rodriguez.

c

The amplicillin-resistance gene has restriction sites for Bam HI and Sal I

d

The restriction site for Pvu II is present in the replication of plasmid (rop) gene.

answer is C.

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Detailed Solution

Tetracycline resistance gene has restriction sites for Bam HI and Sal I sites.

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Which of the following is not a characteristic feature of the vector pBR322?