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Q.

Which of the following is not a component of downstream processing?

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a

Purification

b

Separation

c

Preservation

d

Expression

answer is D.

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Detailed Solution

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Expression is not a component of downstream processing.

Expression of the desired product (r-enzyme or r-protein) is part of upstream processing, which is the first segment of bioprocessing. 

(1) Downstream processing involves separation, extraction and purification of the product. So, separation is the first step of downstream processing.

(2) In downstream processing, purification comes after separation of the product. 

(3) Addition of preservatives is part of formulation of the purified product. The goal of formulation is to stabilise the product and extend its shelf life.

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