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Q.

Which of the following is not a significance of PCR ?

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a

Multiplication of selected gene of interest

b

Early detection of pathogen and disease

c

High accuracy without error

d

Ability to work with minuscule amount of starting DNA

answer is C.

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Detailed Solution

The statement "High accuracy without error" is not a correct significance of the Polymerase Chain Reaction (PCR). While PCR is a powerful and widely used molecular biology technique, it is not immune to errors. There can be various sources of errors in PCR, including:

Contamination: Contamination with DNA from other sources can lead to false-positive results.

Primer-dimer formation: Primers used in PCR can sometimes bind to each other and form primer-dimers, which can interfere with the amplification of the target DNA.

Amplification bias: PCR may not amplify all DNA sequences equally, leading to a bias in the representation of certain sequences.

Mutation or Taq polymerase errors: The DNA polymerase used in PCR, such as Taq polymerase, can introduce errors during DNA replication.

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