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Q.

Which of the following is not required for DNA fingerprinting?

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a

Isolation of DNA

b

Hybridisation of DNA

c

Sequencing of DNA 

d

Digestion of DNA

answer is D.

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Detailed Solution

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DNA fingerprinting does not require sequencing of DNA. It involves identification of differences in some specific regions (e.g., VNTR) in DNA. 
Isolation of DNA from a sample such as blood, saliva, hair, skin cell of the person is the first step in DNA fingerprinting. Digestion of double-stranded DNA is done by restriction endonucleases. Hybridisation of single-stranded DNA with VNTR probe (DNA-DNA hybridisation) helps detect hybrid DNA fragments by autoradiography.
 

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Which of the following is not required for DNA fingerprinting?