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Q.

Which of the following produces blunt ends?

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a

Hind III and Sma I

b

Hae ll and Sma I 

c

EcoR I and EcoR II

d

Hind II and Sma I 

answer is D.

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Detailed Solution

Sma I identifies CCCGGG sequence and cuts the DNA between C and G to make blunt ends. Hind II also makes blunt ends by making straight cuts between the 3rd and 4th bases of 6 base sequence. 

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