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Which of the following restriction enzyme sites are present in pBR322?

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Hind II, BamH I, Sal I

Pvu II, Pst I, Sal I

EcoR V, Cla I, Pvu II

Pvu I, Hind II, EcoR I

answer is B.

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Detailed Solution

In order to link the alien DNA, the vector needs to have very few, preferably single, recognition sites for the commonly used restriction enzymes. pBR322 has Pvu II, Pst I, Sal I, EcoRI, BamH I, Hind III, Pvu II and Cla I restriction sites.

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