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Q.

Which of the following restriction enzymes don’t have their recognition sequences in selectable markers of cloning vector pBR322?

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a

Pst I

b

Sal I

c

Hind III

d

BamH I

answer is C.

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Detailed Solution

The tetracycline resistant gene has restriction sites for Sal I and BamH I and the ampicillin resistant gene has restriction sites for Pst I and Pvu I. Therefore recognition sequence of Hind III is not present in  in selectable markers of cloning vector pBR322.

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