Which of the following staining techniques are used to locate the lysosomes?

(a) Gamori stain

(b) Bismarck stain

(c) Osmium tetroxide

(d) Janus Green Staining

(a) Gamori stain

The Gamori stain (often spelled Gomori) is the classic histochemical technique used to locate lysosomes within a cell.

Specifically, the Gomori acid phosphatase method is used. This staining technique is designed to detect the activity of acid phosphatase, which is a primary marker enzyme for lysosomes. When the stain is applied, a visible precipitate forms at the sites of high enzyme activity, thereby revealing the location of the lysosomes under a microscope.

The other options are incorrect as they are used for different purposes:

• Bismarck stain: A general biological stain for certain tissues.
• Osmium tetroxide: A stain used to visualize lipids (fats) in electron microscopy.
• Janus Green Staining: A vital stain used specifically to identify mitochondria in living cells.