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Q.

Which of the following tools of recombinant DNA technology incorrectly paired with its use ?

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a

Restriction enzyme – Production of free ends.

b

DNA polymerase – Used in a PCR to amplify.

c

DNA ligase – Creates sticky ends of restriction fragments.

d

 Reverse transcriptase – Production of cDNA from mRNA.

answer is B.

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Detailed Solution

DNA ligase joins two fragments of DNA by forming phosphodiester bonds.

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Which of the following tools of recombinant DNA technology incorrectly paired with its use ?