Which of the following vaccines has been produced from yeast by recombinant DNA technology?

Hepatitis B vaccine was introduced in 1986. It was prepared using DNA recombinant technology using yeast cells Saccharomyces cerevisiae. A plasmid with the HB gene was introduced into the yeast to produce the antigen, and it was used as a vaccine.

Hepatitis A vaccine is produced using tissue cultures.

Hib vaccine is prepared using the polysaccharide capsule that surrounds the bacterium, conjugated to a carrier protein.

Oral polio vaccine is prepared by growing the viral strains in monkey kidney cells.

Among these vaccines, Hepatitis B vaccine is also the only vaccine produced using DNA recombinant technology.