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Q.

Which one of the following is used as buffer in separation of DNA fragments by gel electrophoresis?

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a

Tris Acetate EDTA

b

Benzene 

c

Distilled water

d

Alcohol

answer is A.

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Detailed Solution

Buffer is used in gel electrophoresis to provide ions for conduction of electricity and to maintain the pH in the gel matrix. For DNA gel electrophoresis the buffers used are Tris Acetate EDTA, Tris Borate EDTA and Lithium Borate.

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