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Q.

Which one of the following statement is not true regarding gel electrophoresis technique?

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a

The separated DNA fragments are stained by using ethidium bromide.

b

The presence of chromogenic substrate gives blue coloured DNA bands on the gel.

c

Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light.

d

The process of extraction of separated DNA strands from gel is called elution.

answer is B.

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Detailed Solution

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The presence of a chromogenic substrate gives blue coloured colonies if the plasmid in bacteria does not have an insert . Presence of insert do not produce any colour due to inactivation of beta galactosidase gene ,this process is called as insertional inactivation used to identify recombinants from non recombinants .

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