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Q.

Which one of the following steps involved in DNA fingerprinting is based on the negative charge of DNA molecules?

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a

Restriction digestion

b

Autoradiography

c

Gel electrophoresis

d

Southern blotting

answer is B.

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Detailed Solution

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The cutting of DNA by restriction endonucleases results in the fragments of DNA. 

These fragments can be separated by a technique known as gel electrophoresis. 

Since DNA fragments are negatively charged molecules they can be separated by forcing them to move towards the anode under an electric field through a medium/matrix.

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