Which technique is not a part of the methodology employed in DNA fingerprinting?

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Density gradient centrifugation

Gel electrophoresis

Restriction fragmentation

Southern blotting

answer is D.

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Detailed Solution

Density gradient centrifugation is used to separate and purify molecules, such as DNA, RNA, and proteins, based on their size, shape, and density. It is often used in genomic studies to isolate and purify DNA or RNA from complex mixtures. In density gradient centrifugation, a sample is placed in a tube with a gradient of density, which is typically created by adding a layer of a denser material, such as cesium chloride solution, to a layer of a less dense material, such as water or buffer. The sample is then centrifuged, which causes the molecules in the sample to sediment based on their density. As the molecules sediment, they form distinct bands within the gradient, which can then be separated and collected for further analysis.

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