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Q.

Which two enzymes has antagonistic (opposite) activity (bond breaking and linking) in the production of rDNA in genetic engineering ?

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a

Methylase & Taq DNA polymerase

b

Taq DNA polymerase & ligase

c

Restriction endonuclease & ligase

d

Ligase & methylase

answer is B.

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Detailed Solution

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Endonucleases break sugar phosphate back bone and ligase help in the formation of sugar phosphate back bone

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