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Q.

While analysing the DNA of an organism a total number of 5386 nucleotides were found out of which the proportion of different bases were adenine = 29%, guanine = 17%, cytosine = 32%, thymine = 17%. Considering the Chargaff's rule it can be concluded that

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a

it is a double-stranded circular DNA

b

it is single-stranded DNA

c

it is a double-stranded linear DNA

d

No conclusion can be drawn

answer is B.

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Detailed Solution

According to Chargaff's rule of base pairing
i.e., $\frac{A}{T} = \frac{G}{C} = 1$
For the given organisms, the DNA does not follow Chargaff's rule as $\frac{A}{T} = \frac{27}{17} \neq \frac{32}{17} = \frac{G}{C} \neq 1$
Hence, it can be concluded that it is a single-stranded DNA, not double-stranded.

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While analysing the DNA of an organism a total number of 5386 nucleotides were found out of which the proportion of different bases were adenine = 29%, guanine = 17%, cytosine = 32%, thymine = 17%. Considering the Chargaff's rule it can be concluded that