Q.

Why is it advantageous for a vector to have a single recognition site for a restriction enzyme when cloning foreign DNA?  

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a

It enhances the vector’s ability to replicate within host cells.

b

It prevents the loss of any antibiotic resistance genes. 

c

It allows the integration of multiple foreign DNA fragments simultaneously.

d

It simplifies the gene cloning process by avoiding the generation of multiple fragments.

answer is C.

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Detailed Solution

n order to link the alien DNA, the vector needs to have very few, preferably single, recognition sites for the commonly used restriction enzymes. Presence of more than one recognition sites within the vector will generate several fragments, which will complicate the gene cloning.

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