You are given a tissue with its potential for differentiation in an artificial culture. Which of the following pairs of hormones would you add to the medium to secure shoots as well as roots?

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IAA and gibberellin

Auxin and cytokinin

Auxin and abscisic acid

Gibberellin and abscisic acid

answer is B.

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Detailed Solution

Auxins and cytokinins together play a role in callus formation and morphogenesis. When these are in equal concentrations in culture medium, they promote callus formation from explant. High auxin and low cytokinin concentrations promote root formation. High cytokinin to auxin ratio promotes shoot differentiation and leaf formation

(1) IAA, along with gibberellins, has no role in morphogenesis and callus formation. They promote the formation of parthenocarpic fruits.

(3) Auxin and abscisic acid are antagonistic.

(4) Gibberellins and abscisic acids are antagonistic.

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