First slide

Processes of Recombinant DNA Technology

Question

Arrange the following steps for making the bacterial cell competent to take up DNA, in chronological order.
I.    Incubation of cells with recombinant DNA on ice.
II.   Incubation of cells with recombinant DNA at 42 degree Celsius
III.   Treating cells with a specific concentration of a divalent such as calcium

Easy

A

III, I, II, I
B

III, I, II
C

I, III, I, II
D

II, I, III

Solution

In the method of rDNA technology, the bacterial cells must be made competent by treating with divalent cation like Ca. The bacteria along with rDNA must be kept on ice and later for brief period should be treated to 42⁰C temperature and again be kept on ice.

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