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Q.

Arrange the steps of isolation of DNA from the source cell.(a) Precipitation of DNA with chilled ethanol.(b) Treatment of DNA strands with protease and RNase.(c) Treatment of DNA strands with restriction endonuclease.(d) Isolation of DNA from bacterial cells by the treatment of lysozyme.

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a

(d), (a), (b), (c)

b

(b), (a), (d), (c)

c

(d), (a), (c), (b)

d

(d), (b), (a), (c)

answer is D.

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Detailed Solution

The sequential steps in the isolation of gene from the donor organism bacterium is Treating the bacterial cells with lysozymeTreating the protoplasm with protease and RNasePrecipitating the genomic DNA with chilled ethanolSubjecting the DNA with particular Restriction endonuclease treatment.
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