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Plant Growth Regulators
Question

Auxin can be bioassayed by

Easy
Solution

Auxin can be bio-assayed by Avena coleoptile curvature.

Went worked on oat coleoptiles. He placed several decapitated tips of  Avena  coleoptile over a thin block of agar agar. The block was then cut to small pieces and each piece was placed asymmetrically on the cut end of coleoptile. The characteristic bending was observed even in dark. This clearly demonstrated that the substance responsible for growth is synthesized in the apex and translocated downwards into the agar block and finally to one side of decapitated stump. This resulted in unequal growth and thus curvature. The above experiment is a bioassay test for auxins.

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