First slide

Tools of Recombinant DNA Technology

Question

The bacterial cells are made competent by incubating the bacterial cells in:

Easy

A

Polyethylene glycol solution
B

Ethidium bromide solution
C

Calcium chloride solution
D

Sodium nitrate solution

Solution

In order to force bacteria to take up the plasmid, the bacterial cells should first be made ‘competent’ to take up DNA. This is done by incubating the bacterial cells in a specific concentrated calcium salt solution (divalent cation). It renders the bacterial cell wall permeable to the recombinant vectors.

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