First slide

Processes of Recombinant DNA Technology

Question

Bioreactors are involved in

Easy

A

Obtaining the foreign gene product
B

Formation of rDNA
C

Downstream processing
D

Separation of DNA from donor cells

Solution

Bioreactors are huge tanks of 100 to 1000 liters capacity in which optimal conditions are provided for the conversion of raw materials to specific products in large quantities by genetically modified cells or microbes. rDNA is formed from donor DNA and vector DNA by using the enzymes restriction endonuclease and ligase incubated in suitable buffer solution. Separation and purification of product formed in bioreactor is called downstream processing.

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