Characteristic feature of all living cells is presence of

Protoplasm is the physical basis of life. All the bio-chemical activities required to maintain the living state take place in protoplasm. Therefore, protoplasm is the characteristic feature of all living cells. Membrane-bound organelles and well-defined nucleus are found only in eukaryotes and not in prokaryotes. Vacuoles are membrane bound cell organelles present in eukaryotes but absent in prokaryotes.