First slide

Tools of Recombinant DNA Technology

Question

Choose the DNA sequence that is most likely to be cleaved by restriction endonucleases.

Easy

A

5'-ATAACC-3'
3'-TATTGG-5'
B

5'-GCATAA-3'
3'-CGTATT-5'
C

5'-CGATCG-3'
3'-GCTAGC-5'
D

5'-AAGGCC-3'
3'-TTCCGG-5'

Solution

Restriction endonuclease recognises a specific palindromic nucleotide sequences in the DNA. The palindrome in DNA is a sequence of base pairs that reads same on the two strands when orientation of reading is kept the same.

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