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Sulphur is immobile element because it is not easily detached from cell components. Its deficiency symptoms are first seen in younger leaves. Zinc is activator of carboxylases, alcohol dehydrogenase and carbonic dehydrogenase. Anabaena is a cyanobacterium which not only fixes nitrogen to ammonia but also fixes CO2 by photosynthesis. Thiobacillus is a denitrifier that converts nitrates to molecular nitrogen.