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Tools of Recombinant DNA Technology
Question

A cloning vector has tetracycline and ampicillin resistant gene. A foreign gene was inserted in the ampicillin resistant gene. The recombinant would survive in a medium containing:

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Solution

A cloning vector has tetracycline and ampicillin resistant gene. A foreign gene was inserted in the ampicillin resistant gene. The recombinant would survive in a medium containing tetracycline but not ampicillin. This is because of insertional inactivation of the ampicillin resistant gene.

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