First slide

Tools of Recombinant DNA Technology

Question

The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of

Easy

A

insertional in activation of alpha galactosidase in recombinant bacteria.
B

inactivation of glycosidase enzyme in recombinant bacteria
C

non-recombinant bacteria containing beta galactosidase
D

insertional inactivation of alpha galactosidase in non recombinant bacteria.

Solution

A recombinant DNA is inserted within the coding sequence of an enzyme, $β$ -galactosidase. This results into inactivation of the gene for synthesis of this enzyme, which is referred to as insertional inactivation. The presence of a chromogenic substrate gives blue coloured colonies if the plasmid in the bacteria does not have an insert. Presence of insert results into insertional inactivation of the $β$ -galactosidase gene and the colonies do not produce any colour, these are identified as recombinant colonies.

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