First slide

Processes of Recombinant DNA Technology

Question

Competent bacteria are given thermal shock for forcing the entry of rDNA into host bacteria at the following temperature.

Moderate

A

$52^{o} C$
B

$42^{o} C$
C

$62^{o} C$
D

$32^{o} C$

Solution

In rDNA technology, the host bacteria are made competent by treating them with ${CaCl}_{2}$ , which make the rDNA pass through the holes of cell wall and comes in contact with the cell membrane. Cell membrane is impermeable to DNA as DNA is negatively charged. The host bacterial cells are incubated with rDNA on ice or at low temperatures followed by placing them briefly at $42^{o} C$ (heat shock) and then putting them back on ice. This makes the bacteria taking up the recombinant DNA.

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