First slide

Tools of Recombinant DNA Technology

Question

A competent cell is defined as cells:

Easy

A

Those which have been given a heat shock at $42^{0}$ C for 45 seconds
B

Those which has been treated with calcium chloride
C

The cells that show susceptibility to tetracyclin
D

The cell that are resistant to ampicillin

Solution

In order to force bacteria to take up the plasmid, the bacterial cells must first be made ‘competent’ to take up DNA. This is done by treating them with a specific concentration of a divalent cation, such as calcium, which increases the efficiency with which DNA enters the bacterium through pores in its cell wall.

Get Instant Solutions

When in doubt download our app. Now available Google Play Store- Doubts App

Recieve an sms with download link

Doubts App

OR

SCAN ME

Doubts App

Doubts App